Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
نویسندگان
چکیده
منابع مشابه
Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes.
Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both. Here we present a novel method of amplification detection that harnesses the pH change resulting from amplification reactions performed with minimal buffering capacity....
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2015
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114253